The Prem table if VAR errors hadn’t cheated Utd, City, Spurs and Co out of points

first_imgMANCHESTER UNITED, City and Tottenham are just a few of the teams hard done by with VAR decisions this season.While the video replay system has cleared up doubt with a number of key decisions, the pendulum has violently swung between pernickety fiddling on minor infringements and blasé shrugs at clear fouls.3 SunSport’s Martin Lipton reviewed every major VAR decision – and non-decisions – so far this season before making his call on what SHOULD have happened. This is how the table would look if those calls had been made.Stonewall penalties such as Jamal Lascelles’ cleverly disguised pull down on Harry Kane should have been caught by the slow-motion review, which has preferred to crack the whip on attackers flicking the ball as it pings across the box.Offsides are now measured by the millimetre to ensure a very exact level of fairness – hard to argue with in theory but a little tedious in the heat of the moment.VAR OVERTURNSWest Ham v Man City: Jesus goal disallowed, Aguero missed penalty retaken and scoredScore without VAR: 0-5Leicester v Wolves: Dendoncker goal disallowedScore without VAR: 0-1Brighton v West Ham: Trossard goal disallowedScore without VAR: 2-1Man City v Spurs: Jesus goal disallowedScore without VAR: 3-2Norwich v Chelsea: Zouma goal disallowedScore without VAR: 2-4The most momentous decision in the opening four weeks saw Manchester City have a late winner over Spurs ruled out because of Aymeric Laporte’s acute handball.Brighton and Wolves have also had winning goals taken off them, while strikes to make victories more comfortable were stripped off City and Chelsea.But it is the unfairly missed decisions that are most galling.The ‘clear and obvious’ error rule with penalty fouls is being interpreted with incredibly high standards.MISSED VAR OVERRULESMan City v Spurs: Lamela concedes penalty for Rodri foulAdjusted score: 3-2Norwich v Chelsea: Stiepermann foul on Azpilicueta is a penaltyAdjusted score: 2-4Man Utd v C Palace: Martial hauled down by Kelly in boxAdjusted score: 2-2Bournemouth v Man City: Lerma clear foul on SilvaAdjusted score: 2-4Spurs v Newcastle: Lascelles brings down Kane in boxAdjusted score: 1-1West Ham v Norwich: Clear foul on Haller in the boxAdjusted score: 3-0Newcastle v Watford: Handball in lead-up to Newcastle equaliser missed by VARAdjusted score: 0-1Erik Lamela has been allowed to grab Rodri by the neck as a corner came in and Martin Kelly able to manhandle Anthony Martial as he aimed to shoot.Such was the ferocity of the foul on City midfielder Rodri, Pep Guardiola remarked that VAR had “gone for a coffee” at that point of the 2-2 draw.But the boot was on the other foot as Spurs’ equalising penalty versus Newcastle went by the wayside, showing that perhaps VAR luck will equal itself out over the season – just as we have always told ourselves regular decisions do.3Latest Football newsSILVA’S GOLDEN GIRLModel Ines Tomaz has been helping Bernardo Silva through quarantineGossipCROWD RETURNFA Cup final ‘may see 20,000 at Wembley in trial allowing some fans at games’CommentPHIL THOMASDiving and whining was never a good look and will seem worse after lockdownExclusive’I’M IN A BAD WAY’Ex-England star Kenny Sansom talks for first time since being attackedExclusiveBOURNE AGAINHowe says Cherries are stronger after lockdown with FIVE fit-again starsExclusiveSWAN THAT GOT AWAYSwansea wanted £3m Davies three years ago but couldn’t get work permitNEXT STEPJonny Hayes set to move to English Championship having been let go by CelticBAYERN 5 DUSSELDORF 0Lewa bags two as Bayern thump rivals to close on 8th straight titleLADBROKES 1-2-FREE Simply predict England, Wales and Scotland vs Belgium scores this weekend and win £100Something must soon be about to come back and bite the Magpies then, as Isaac Hayden’s handball before the leveller versus Watford was missed due to “human error”.It shows how a major flaw with VAR isn’t so much the fact we have replays but the fact humans are still in charge of them.The system still needs to develop and is certainly not going to go away – let’s just hope there is a plan to fix the kinks rather than simply being told to live with them.3 United were left furious by the decision when trailing to PalaceCredit: ReutersPep Guardiola hits out at VAR in 90 second press conference as Manchester City miss out on another penaltylast_img read more

One of our reporters tried to do CRISPR He failed miserably

first_img Country * Afghanistan Aland Islands Albania Algeria Andorra Angola Anguilla Antarctica Antigua and Barbuda Argentina Armenia Aruba Australia Austria Azerbaijan Bahamas Bahrain Bangladesh Barbados Belarus Belgium Belize Benin Bermuda Bhutan Bolivia, Plurinational State of Bonaire, Sint Eustatius and Saba Bosnia and Herzegovina Botswana Bouvet Island Brazil British Indian Ocean Territory Brunei Darussalam Bulgaria Burkina Faso Burundi Cambodia Cameroon Canada Cape Verde Cayman Islands Central African Republic Chad Chile China Christmas Island Cocos (Keeling) Islands Colombia Comoros Congo Congo, the Democratic Republic of the Cook Islands Costa Rica Cote d’Ivoire Croatia Cuba Curaçao Cyprus Czech Republic Denmark Djibouti Dominica Dominican Republic Ecuador Egypt El Salvador Equatorial Guinea Eritrea Estonia Ethiopia Falkland Islands (Malvinas) Faroe Islands Fiji Finland France French Guiana French Polynesia French Southern Territories Gabon Gambia Georgia Germany Ghana Gibraltar Greece Greenland Grenada Guadeloupe Guatemala Guernsey Guinea Guinea-Bissau Guyana Haiti Heard Island and McDonald Islands Holy See (Vatican City State) Honduras Hungary Iceland India Indonesia Iran, Islamic Republic of Iraq Ireland Isle of Man Israel Italy Jamaica Japan Jersey Jordan Kazakhstan Kenya Kiribati Korea, Democratic People’s Republic of Korea, Republic of Kuwait Kyrgyzstan Lao People’s Democratic Republic Latvia Lebanon Lesotho Liberia Libyan Arab Jamahiriya Liechtenstein Lithuania Luxembourg Macao Macedonia, the former Yugoslav Republic of Madagascar Malawi Malaysia Maldives Mali Malta Martinique Mauritania Mauritius Mayotte Mexico Moldova, Republic of Monaco Mongolia Montenegro Montserrat Morocco Mozambique Myanmar Namibia Nauru Nepal Netherlands New Caledonia New Zealand Nicaragua Niger Nigeria Niue Norfolk Island Norway Oman Pakistan Palestine Panama Papua New Guinea Paraguay Peru Philippines Pitcairn Poland Portugal Qatar Reunion Romania Russian Federation Rwanda Saint Barthélemy Saint Helena, Ascension and Tristan da Cunha Saint Kitts and Nevis Saint Lucia Saint Martin (French part) Saint Pierre and Miquelon Saint Vincent and the Grenadines Samoa San Marino Sao Tome and Principe Saudi Arabia Senegal Serbia Seychelles Sierra Leone Singapore Sint Maarten (Dutch part) Slovakia Slovenia Solomon Islands Somalia South Africa South Georgia and the South Sandwich Islands South Sudan Spain Sri Lanka Sudan Suriname Svalbard and Jan Mayen Swaziland Sweden Switzerland Syrian Arab Republic Taiwan Tajikistan Tanzania, United Republic of Thailand Timor-Leste Togo Tokelau Tonga Trinidad and Tobago Tunisia Turkey Turkmenistan Turks and Caicos Islands Tuvalu Uganda Ukraine United Arab Emirates United Kingdom United States Uruguay Uzbekistan Vanuatu Venezuela, Bolivarian Republic of Vietnam Virgin Islands, British Wallis and Futuna Western Sahara Yemen Zambia Zimbabwe Roland Wagner, a postdoc in the lab of Sumit Chanda at the Sanford Burnham Prebys Medical Discovery Institute in San Diego, California, agreed to serve as my CRISPR sensei. An experienced rock climber originally from Austria, Wagner approaches everything methodically. He pulls up the sequence of the CD32 gene, which has five distinct protein-coding regions. If we cut the DNA in one region, the gene most likely would be knocked out: It would no longer make its protein. Now, at Wagner’s lab bench, I face a rack of fancy plastic gizmos that look like squirt guns but enable users to suck up precise microliters of liquid with a push of a button. My task is to pipette the oligo from one tiny test tube into another. The second tube holds a plasmid, which is a circular piece of DNA that will act as a Trojan horse. This plasmid, customized for CRISPR experiments, already holds the gene for Cas9. It also contains a 60-nucleotide “hairpin” sequence that ultimately will attach to the 20 nucleotides I add to make the full gRNA.I use one of the fancy pipettes to move the oligo into the plasmid tube, and I also add buffer, water, and an enzyme. If all goes well, the enzyme will cut open the plasmid, removing a piece of its DNA and allowing the oligo to take its place. All does not go well.”Oops!” Wagner says as I pipette the enzyme. “You failed a little bit.” I apparently hit the pipette button before submerging the tip into the liquid.In the end, I manage the procedure. After waiting for the chemical reactions to take place, we take my CRISPR plasmid to an electrophoresis machine, a tray that has wires hooked to it. We add a liquid that quickly turns to gelatin, and then I pipette a few drops of my CRISPR plasmid into different lanes on the device. I flip a switch to apply an electric current, which should separate the DNA into bands based on weight. The small piece of DNA I cut out with the enzyme should form a distinct band.My gel electrophoresis only has one band, from the plasmid.”It doesn’t look like it worked,” Wagner says gently. “I didn’t want to be all picky, but it could be that you messed up the enzyme with your pipetting.” He allows that when he was starting out, his experiments often failed. “I’d go home and I’d say, ‘I hate my life,'” he confided. “There are a lot of setbacks in science.”I’ve already learned that any idiot cannot do CRISPR: It takes, at least, basic laboratory skills.Wagner conducts the experiment in parallel with me, and his plasmid properly incorporates the oligo that will guide Cas9 to its target. We then coax a cell line made from an embryonic kidney into taking up the Trojan horse plasmid. After a few days, we isolate the DNA from the cells, amplify it with the polymerase chain reaction, and use electrophoresis to show that the CD32 gene has been cut into pieces. Voilà, our knockout worked.”You did great,” Wagner tells me. “Way to go!”I did not do great. But CRISPR did its job. Sign up for our daily newsletter Get more great content like this delivered right to you! Country By Jon CohenNov. 3, 2016 , 10:00 AM CRISPR uses a guide made of RNA to direct molecular scissors—part of the CRISPR-associated protein, or Cas9—to exact spots in a genome. We could buy the guide RNA (gRNA), but the idea appalls Wagner. “I would assume it’s probably $500 to buy the gRNA, but I wouldn’t know,” he says. “We’re making our own and we’re spending about $5.”The gRNA sequence must complement a stretch of 20 nucleotides on the segment of the CD32 gene we want to cut. But the same DNA sequence could recur elsewhere in the genome, leading the molecular scissors to cut in the wrong place. Such “off-target” effects can cause mayhem, and eliminating them is a key goal of those honing their CRISPR skills. To make the match more specific, Cas9 requires an additional sequence flanking the targeted 20 nucleotides: N-G-G, in which “N” can be any nucleotide. Where Cas9 finds N-G-G immediately following the 20 nucleotides, it attaches to and opens the double helix, allowing the gRNA to bind. Cas9 then cuts each strand of the DNA.To homebrew our gRNA, Wagner copies the sequence of the CD32 segment we’ve identified and pastes it into a freely available database, Optimized CRISPR Design, that looks for a matching set of 20 nucleotides followed by N-G-G. There are 41 options within CD32. The database scans the entire human genome to see whether there are identical matches elsewhere—potential sites of off-target cuts. We select a sequence that appears unique, and then he goes to another website and orders a stretch of DNA—an oligonucleotide—with that sequence.The oligo arrives, and I lose my modern pipetting virginity. I have not worked in a lab since I was an undergraduate more than 30 years ago. Back then, I learned a pipetting technique that probably was invented by Louis Pasteur: I put a finger in my mouth and then sucked up a chemical into a thin glass tube, capping it with my fingertip when I had drawn up enough.center_img Click to view the privacy policy. Required fields are indicated by an asterisk (*) I speak biology fluently, but the molecular complexities of the novel genome-editing tool called CRISPR left me as befuddled as when I peruse descriptions of the inflationary universe. So I decided to test what one investigator told me: CRISPR (for “clustered regularly interspaced short palindromic repeats”) may sound intimidating, but it is so simple to use that “any idiot” could do it.I would give it a try.CRISPR works best at crippling, or knocking out, genes, so that’s how I choose to use it. But I aim high: I target an immune gene that, I theorize, could lead to insights into reducing the harm done by Zika virus. (My admittedly wild hypothesis is that the gene, CD32, may help drive Zika virus to copy itself to higher levels if a person was previously infected with dengue and has antibodies to that virus.) One of our reporters tried to do CRISPR. He failed miserably Emaillast_img read more